51986

A general introduction to the principles of stem cell biology. Topics include embryonic stem cells in early development, adult stem cells, and potential applications of stem cell culture and ethical issues involved in stem cell research. Current research methods involving cell differentiation and fluorescent technology will be presented. Emphasis on laboratory techniques including culture of mouse embryonic stem cells, analysis of stem cells by immunofluorescence and flow cytometry.

Introduction to techniques for culturing and maintaining mammalian cells, including proper use of equipment, aseptic technique, media formulation, storage, counting and subculturing of cells. Contents include proper growth conditions, cell cycle regulation, cellular responses to DNA damage, growth patterns, viability assays, transfection and an introduction to the use of fluorescent molecules in visualization of cellular structures.

In-depth coverage of current recombinant DNA methods and concepts of modern genomics. Emphasis on laboratory work using techniques such as culture of bacteria, DNA and RNA purification, plasmid DNA construction, cDNA synthesis, real-time PCR, and use of software tools to analyze DNA sequences.

The theory and practice of current polymerase chain reaction (PCR) analytical methods. Laboratory experience in techniques such as multiplex PCR, reverse transcriptase PCR (RT-PCR), and real-time PCR. Experimental optimization is emphasized.

Students will explore various careers in bioscience through online research, informational interviews and interactions with industry professionals. Students will present a summary of a chosen bioscience topic at a scientific meeting. Students will communicate and network with industry professionals, and attend professional workshops and seminars to gain career skills needed for finding internships and jobs in the biotechnology fields.

Underlying principles of immunoassay with focus on Western blots. Discussion of applications of Western blotting techniques used in the pharmaceutical, biotechnology, and clinical laboratory settings. Laboratory exercises feature hands-on exercises emphasizing all aspects of Western blotting, including running protein gels, blotting, immunodetection, and data analysis.

Introduction to the principles and techniques of molecular and cell biology and protein analysis. Emphasis on lab techniques, such as spectroscopy, preparation of cell lysates, Western blot, immunoprecipitation, enzyme assays, analysis of signal transduction, and stable transfection of mammalian cells. Design and analysis of molecular and cell biology experiments is taught.

Introduction to the theoretical aspects and laboratory techniques of the Polymerase Chain Reaction (PCR). Practical experience performing PCR as well as experimenting with optimization of the reaction. Discussion of applications of PCR used in biotechnology industry, basic science, forensics, epidemiology, diagnostics, and determination of evolutionary relationships.

Development of complex analysis and enhanced scientific presentation skills necessary for continuing students placed in off-campus stem cell biology internships. Students will complete and present a novel research project. Types of internships may include, but are not limited to, differentiation of pluripotent stem cells, FACS, cell culture, propagation of iPS cells, immunolocalization, micro array analysis and tumor cell characterization.

Support for student interns placed in local bioscience laboratories. Provide tools to effectively communicate internship experience, present scientific concepts and network with professionals in the field. Types of internships may include, but are not limited to, basic research, biomanufacturing, quality control, environmental sciences, and food sciences.