ADVISE: Readiness for college-level English or ESL 188
An introduction to the growing field of biotechnology by pairing practical hands-on laboratory activities with career presentations by scientists and industry professionals. Basic biological concepts are covered along with discussions of careers in biomanufacturing, forensics, agriculture, biofuels, bioinformatics and drug discovery.
Career professionals in biotechnology and related fields will discuss their current bioscience programs and provide information on career opportunities and pathways. Presentations will cover basic research, new products, processes, and prospects for the future in medicine, environmental restoration, forensics, and agriculture. Implications for society and governmental regulations will also be discussed.
PREREQ: Completion of high school general Biology course
Introduction to mammalian cell culture and stem cell biology and techniques. Practical hands-on experience includes aseptic technique, counting cells, cell/stem cell culture maintenance, fluorescence labeling, and stem cell differentiation.
COREQ: BTEC 107 and BTEC 108A ADVISE: (ESLN 3700 or ESL 182) or (placement into ESLN 3800 or ESL 184)
Introduction to the laboratory skills and concepts necessary to work in the biotechnology industry, allied health or other biology-related fields. This course is part of the learning community Bridge to Biosciences program.
ADVISE: ESL 186 or placement in ESL 188 or readiness for college-level English
A general overview of Food and Drug Administration regulations as they pertain to the biotechnology field. Knowledge of Current Good Laboratory Practices (cGLP) and Current Good Manufacturing Practices (cGMP) is needed to work in biotechnology manufacturing and preclinical research laboratories. The course will emphasize those practices as they pertain to the biopharmaceutical industry.
ADVISE: BIO 11 and (ESL 188 or readiness for college-level English; Completion of or concurrent enrollment in: BTEC 108A
An introduction to laboratory techniques needed for entry-level positions in the biotechnology industry and research laboratories. The course covers basic lab skills such as solution making, bacteria cultures, plasmid DNA and protein purification. Intended as an introductory class for the students in preparation of the more advanced biotechnology classes and/or an internship in a biotechnology lab.
PREREQ: BTEC 15 and completion of 4 units of BTEC classes with wet-lab components with a grade of C or better. The students may choose from these classes: BTEC 14A, BTEC 20, BTEC 21C, BTEC 22, BTEC 23, BTEC 24, BTEC 25, BTEC 103, BTEC115 and BTEC 120. COREQ: BTEC 93
Support for student interns placed in local bioscience laboratories. Provide tools to effectively communicate internship experience, present scientific concepts and network with professionals in the field. Types of internships may include, but are not limited to, basic research, biomanufacturing, quality control, environmental sciences, and food sciences.
Instructor approval and completion of minimum of 4 units of CCSF BTEC classes with wet-labs is required.
ADVISE: Readiness for college-level English or ESL 188; Completion of or concurrent enrollment in: Any biotechnology laboratory course at CCSF
Students will explore various careers in bioscience through online research, informational interviews and interactions with industry professionals. Students will present a summary of a chosen bioscience topic at a scientific meeting. Students will communicate and network with industry professionals, and attend professional workshops and seminars to gain career skills needed for finding internships and jobs in the biotechnology fields.
ADVISE: BIO 11; BTEC 108A; Completion of or concurrent enrollment in: CHEM 32
Introduction to techniques for culturing and maintaining mammalian cells, including proper use of equipment, aseptic technique, media formulation, storage, counting and subculturing of cells. Contents include proper growth conditions, cell cycle regulation, cellular responses to DNA damage, growth patterns, viability assays, transfection and an introduction to the use of fluorescent molecules in visualization of cellular structures.
ADVISE: ((ET 108A or (elementary algebra or higher)) and BIO 11 and (CHEM 32 or CHEM 40)
Introduction to techniques for culturing mammalian cells, including media formulation, aseptic technique, freezing, thawing, subculturing, and maintaining cells. Theory includes maintaining proper growth conditions, preventing contamination, as well as cellular responses to DNA damage and gene expression. Practical experience includes the proper use and care of equipment for culturing cells and performing cell growth and viability assays.
PREREQ: BTEC 21A
Introduction to advanced techniques in the study of normal and mutant tissue culture cells including organelle visualization with various fluorophores, transfection with fluorescent markers, and immunostaining. In depth coverage of the theory behind and use of fluorescent microscopy and current research methods using fluorescent technology, including apoptosis assays and immunocytochemistry, DNA microarrays and FACS analysis.
PREREQ: BTEC 21B or BTEC 20
A general introduction to the principles of stem cell biology. Topics include embryonic stem cells in early development, adult stem cells, and potential applications of stem cell culture and ethical issues involved in stem cell research. Current research methods involving cell differentiation and fluorescent technology will be presented. Emphasis on laboratory techniques including culture of mouse embryonic stem cells, analysis of stem cells by immunofluorescence and flow cytometry.
PREREQ: BTEC 21C COREQ: BTEC 93
Provides the necessary communication tools for beginning students placed in off-campus stem cell biology internships. Emphasis is put on demonstrating how their work contributes to the overall the scientific research being conducted at their internship site. Types of internships may include, but are not limited to, differentiation of pluripotent stem cells, FACS analysis, primary cell culture, propagation of iPS cells, immunolocalization, microarray analysis and tumor cell characterization.
PREREQ: BTEC 21D COREQ: BTEC 93
Development of complex analysis and enhanced scientific presentation skills necessary for continuing students placed in off-campus stem cell biology internships. Students will complete and present a novel research project. Types of internships may include, but are not limited to, differentiation of pluripotent stem cells, FACS, cell culture, propagation of iPS cells, immunolocalization, micro array analysis and tumor cell characterization.
ADVISE: BIO 11 and (elementary algebra or higher)
Underlying principles of immunoassay with focus on ELISA. Examples of ELISA techniques and applications in the pharmaceutical, environmental, biotechnology, and clinical laboratory settings will be discussed. Laboratory exercises and discussions cover qualitative and quantitative data analysis and direct, indirect, sandwich, and competitive ELISA methods.
ADVISE: BIO 11 and ((BTEC 108A or (elementary algebra or higher))
Underlying principles of immunoassay with focus on Western blots. Discussion of applications of Western blotting techniques used in the pharmaceutical, biotechnology, and clinical laboratory settings. Laboratory exercises feature hands-on exercises emphasizing all aspects of Western blotting, including running protein gels, blotting, immunodetection, and data analysis.
ADVISE: BIO 11 and ((BTEC 108A or (elementary algebra or higher))
Introduction to the theoretical aspects and laboratory techniques of the Polymerase Chain Reaction (PCR). Practical experience performing PCR as well as experimenting with optimization of the reaction. Discussion of applications of PCR used in biotechnology industry, basic science, forensics, epidemiology, diagnostics, and determination of evolutionary relationships.
PREREQ: BTEC 24 or demonstration of BTEC 24 exit skills
The theory and practice of current polymerase chain reaction (PCR) analytical methods. Laboratory experience in techniques such as multiplex PCR, reverse transcriptase PCR (RT-PCR), and real-time PCR. Experimental optimization is emphasized.
PREREQ: Approval of the Engineering and Technology Department COREQ: BTEC 14B or BTEC 21D or BTEC 21E
Work experience for students in the Biotechnology Program through internships in the biotechnology industry. Students do internships in local academic/government institutions and biotechnology companies. One unit of credit is earned for 60 hours of unpaid, or 75 hours of paid internship.
ADVISE: CHEM 32 and (BIO 11 or BTEC 10) and BTEC 120
An introduction to protein purification techniques including sample preparation, column chromatography, and analysis of purification. Hands on training with manual and automated chromatography systems used in industry and research laboratories. Purification analysis includes gel electrophoresis, specific activity, and mass balance calculations.
COREQ: BTEC 10 and BTEC 108A ADVISE: (ESLN 3700 or ESL 182) or (placement in ESLN 3800 or ESL 184
Introductory level lecture course covering basic scientific language and concepts of biology and chemistry, and the academic study skills needed to succeed in science courses. An orientation to the field of biotechnology and professional opportunities.
BTEC 108A is part of a learning community for Bridge to Biosciences. Concurrent enrollment in BTEC 10 and BTEC 107 is required. For more info, contact Li Lovett by email: email@example.com
ADVISE: (ESLN 3700 or placement into ESLN 3800) and (elementary algebra or higher)
Concepts, techniques and applications of mathematics and elementary algebra emphasizing applications to practical problems in biotechnology and chemistry. Use of traditional problem solving methods and interactive group activities.
ADVISE: BIO 11 and CHEM 32
In-depth coverage of current recombinant DNA methods and concepts of modern genomics. Emphasis on laboratory work using techniques such as culture of bacteria, DNA and RNA purification, plasmid DNA construction, cDNA synthesis, real-time PCR, and use of software tools to analyze DNA sequences.
Formerly BIO 65 Offered Fall semesters
ADVISE: BIO 11 and (CHEM 32 or CHEM 40)
Introduction to the principles and techniques of molecular and cell biology and protein analysis. Emphasis on lab techniques, such as spectroscopy, preparation of cell lysates, Western blot, immunoprecipitation, enzyme assays, analysis of signal transduction, and stable transfection of mammalian cells. Design and analysis of molecular and cell biology experiments is taught.