BTEC 25 Analytical PCR Technology (1)
Lec-16, Lab-8, field trips
Credit, Degree Applicable
PREREQ.: Completion of BTEC 24
or demonstration of BTEC 24
A course in the theory and practice of current polymerase chain reaction (PCR) analytical methods. Students will gain laboratory experience in performing techniques such as reverse transcriptase PCR (RT-PCR), real-time PCR, inverse PCR, construction of oligonucleotide DNA microarrays, site-directed mutagenesis, and strategies for cloning genes from organisms lacking genome projects. Experimental optimization will be emphasized.
After successful completion of this course, students will be able to:
- Outcome 1: Relate the appropriate types of PCR methods to solving current experimental problems in molecular biology.
- Outcome 2: Describe the underlying theory of PCR methods such as RT-PCR and real-time PCR.
- Outcome 3: Design an acceptable protocol utilizing available PCR methods to solve an experimental or biological problem.
- Outcome 4: Utilize Internet websites available for primer design, genome sequences, and reaction optimization.
- Outcome 5: Set up an appropriate PCR reaction.
- Outcome 6: Operate and program a thermocycler to accomplish a particular protocol.
- Outcome 7: Interpret and analyze experimental PCR results.