BTEC 25 Analytical PCR Technology (1)
Lec-16, lab-8 (total hrs), field trips
Credit, Degree Applicable
PREREQ.: Completion of BTEC 24
or demonstration of BTEC 24
A course in the theory and practice of current polymerase chain reaction (PCR) analytical methods. Students will gain laboratory experience in performing techniques such as reverse transcriptase PCR (RT-PCR), real-time PCR, inverse PCR, construction of oligonucleotide DNA microarrays, site-directed mutagenesis, and strategies for cloning genes from organisms lacking genome projects. Experimental optimization will be emphasized.