BTEC 24 Introduction to PCR (1)
Lec-16, Lab-8, field trips
Credit, Degree Applicable
An introduction to the theoretical aspects and laboratory techniques of the Polymerase Chain Reaction (PCR). Students gain practical experience performing PCR as well as experimenting with optimization of the reaction. Applications of PCR used in DNA fingerprinting, diagnostic medicine, genome mapping, evolutionary relationship determination, and epidemiology will be discussed.
After successful completion of this course, students will be able to:
- Outcome 1: Relate DNA structure and DNA replication to PCR.
- Outcome 2: Determine the final concentration and volumes necessary for each sample reaction by using dimensional analysis and other mathematical calculations
- Outcome 3: Use positive and negative controls properly in experimental conditions.
- Outcome 4: Set up a PCR
- Outcome 5: Interpret and evaluate electrophoresis gels and analyze the results of a PCR experiment.
- Outcome 6: Operate and program a thermocycler.
- Outcome 7: Relate PCR to practical applications in forensics, epidemiology, diagnostics, and determination of evolutionary relationships.
- Outcome 8: Identify possible sources of contamination and limitations of PCR.