BTEC 23 Western Blotting Techniques (1)
Lec-16, Lab-8, field trips
Credit, Degree Applicable
Underlying principles of immunoassays with focus on Western blotting. Examples of new procedures, technical advances, past, present and future prospects in the pharmaceutical, biotechnology and clinical laboratory settings will be discussed. Implications for society and governmental regulations will be addressed. Course will feature hands-on laboratory exercises emphasizing all aspects of Western blotting.
After successful completion of this course, students will be able to:
- Outcome 1: Relate theoretical principles to laboratory techniques used in Western blotting.
- Outcome 2: Describe biological concepts and use terminology related to Western blotting.
- Outcome 3: Identify and evaluate ethical issues associated with the use of Western blots in biotechnology, pharmaceutical and clinical laboratories.
- Outcome 4: Apply and assess knowledge of Western blots in making educational and career choices.
- Outcome 5: Demonstrate technical skills in care, use, and maintenance of laboratory equipment used for Western blotting: electrophoresis chambers, power, supplies, and transblotting chambers.
- Outcome 6: Calibrate, maintain, and properly use micropipettors.
- Outcome 7: Apply molecular weight standards and controls in Western blotting laboratory experimental design and analysis.