BTEC 21C Stem Cell Technology (3)
Lec-48, Lab-24, field trips
Credit, Degree Applicable
A general introduction to the principles of stem cell biology. Topics include embryonic stem cells in early development, adult stem cells, potential applications of stem cell culture and ethical issues involved in stem cell research. Emphasis on laboratory techniques including proper use and care of equipment, flow cytometry, isolation of mouse adult stem cells, and culture of mouse embryonic stem cells.
After successful completion of this course, students will be able to:
- Outcome 1: Relate principles of stem cell biology to proper laboratory techniques.
- Outcome 2: Critically and independently assess the reality and future of applied stem cell biology.
- Outcome 3: Differentiate between different types of stem cells in terms of their function, use and care.
- Outcome 4: Differentiate adult stem cells into myocytes and osteoblast cells.
- Outcome 5: Perform immunocytochemistry on stem cells and differentiated cells.
- Outcome 6: Describe the underlying principles of flow cytometry.
- Outcome 7: Use a FACScan (flow cytometer).
- Outcome 8: Analyze cell surface markers and cell types using a FACScan.
- Outcome 9: Handle and care for embryonic stem (ES) cells while maintaining their pluripotency.
- Outcome 10: Properly culture and maintain feeder cells.
- Outcome 11: Use proper culture techniques to induce differentiation of ES cells into different cell types.
- Outcome 12: Perform karyotyping of ES cells.
- Outcome 13: Properly freeze and thaw stem cells to maintain viability.
- Outcome 14: Relate cultural and social attitudes to ethical issues and governmental policies concerning stem cell research