BTEC 21B Fluorescent Cell Technology (2)
Lec-36, Lab-18, field trips
Credit, Degree Applicable
Introduction to advanced techniques in the study of normal and mutant tissue culture cells including organelle visualization with various fluorophores, transfection with fluorescent markers, and immunostaining. In depth coverage of the theory behind and use of fluorescent microscopy and current research methods using fluorescent technology, including apoptosis assays and immunocytochemistry, DNA microarrays and FACS analysis.
TITLE 5 CLASSIFICATION CREDIT/DEGREE APPLICABLE (meets all standards of Title 5. Section 55002(a))
After successful completion of this course, students will be able to:
- Outcome 1: Analyze and discuss experimental data in industry-standard laboratory notebook format.
- Outcome 2: Evaluate fluorescence theory and apply to cell biology research.
- Outcome 3: Describe proper and safe operation of fluorescent microscopy lab equipment to stain and label cells/organelles.
- Outcome 4: Analyze performed cellular assays using fluorescent staining.