BTEC 115 Recombinant DNA Biotechnology (5)
Lec-52.5, Lab-105, field trips
Credit, Degree Applicable
An in-depth coverage of recombinant DNA/genetic engineering concepts and principles with a strong emphasis on the laboratory procedures involved in DNA manipulation.
Formerly BIO 65
Offered Fall semesters
After successful completion of this course, students will be able to:
- Outcome 1: Define and use SI units to measure mass, length, volume, temperature, etc
- Outcome 2: Properly utilize the materials and properly operate the standard laboratory equipment used in normal recombinant DNA manipulation techniques.
- Outcome 3: Apply the principles of proper documentation handing in experiment data collection and record keeping.
- Outcome 4: Apply critical thinking in data analysis including statistical and graphical analysis, and in the interpretation of the experimental results.
- Outcome 5: Employ proper methods of laboratory report development and presentation including use of a computer for data analysis and report generation.
- Outcome 6: Utilize proper techniques of bacterial culture including sterile technique, serial dilution, and culture and fermentation as used in recombinant DNA science procedures.
- Outcome 7: Describe and apply techniques of recombinant DNA biotechnology such as plasmid isolation and purification, restriction enzyme digestion, evaluation of the success of a restriction enzyme digestion, DNA ligation, transformation, and other standard techniques in a step-wise manner.
- Outcome 8: Describe and apply techniques of DNA cloning, genomic library and screening techniques, DNA sequencing techniques including colony hybridization, preparation and use of bioassays, blot techniques, RFLP's and DNA fingerprinting.
- Outcome 9: Evaluate and compare the importance and applications of recombinant DNA science in medical industrial, and agricultural practices, and in human genetics including genetic screening, gene therapy, identification by DNA fingerprinting techniques, and the Human Genome Project.
- Outcome 10: Assess the ethical aspects of recombinant DNA science and the regulatory controls involved.