BTEC 101 Fermentation and Protein Purification (5)
Lec-70, Lab-70, field trips
Credit, Degree Applicable
Skills needed to become a technician in biotechnology production. Students grow and monitor cultures emulating large-scale production used in industry. Cleaning, sterilization, aseptic inoculation, operation, and monitoring of fermenters and bioreactors, Recover and purify proteins from cell cultures. Current Good Manufacturing Practices (cGMP) and Standard Operating Procedures (SOP) are emphasized.
Offered on occasion
After successful completion of this course, students will be able to:
- Outcome 1: Work in teams.
- Outcome 2: Generate new ideas, individually or as a group.
- Outcome 3: Formulate alternative solutions.
- Outcome 4: Design a plan of action.
- Outcome 5: Interpret data in all formats.
- Outcome 6: Develop a learning environment.
- Outcome 7: Distinguish the basic biological concepts that underlie the growth characteristics of bacterial, yeast, and mammalian cells.
- Outcome 8: Select and properly use the appropriate instrumentation required to prepare materials for cell culture.
- Outcome 9: Select and properly use the appropriate instrumentation required to monitor cell culture parameters.
- Outcome 10: Relate biochemical concepts to the function of bioreactor components.
- Outcome 11: Grow cells in a bioreactor.
- Outcome 12: Analyze the basic concepts behind the techniques used to separate proteins.
- Outcome 13: Select and properly use the instrumentation required to measure buffer conditions and protein quantity.
- Outcome 14: Relate biochemical concepts to protein separation techniques.
- Outcome 15: Set up current Good Manufacturing practices (cGMP) principles by following Standard Operating Procedures (SOP) and keeping records in Batch Production Record (BPR) format.
- Outcome 16: Prepare results and analysis in written and seminar format