Chemistry Instrumentation at City College
City College chemistry students have hands-on access to a wide
range of analytical instruments, many of which are not available to
lower-division students at four-year institutions. Here are some of
the instrumental methods that are used by students in our
courses:
- Nuclear Magnetic Resonance (NMR): uses the magnetic
properties of atomic nuclei to obtain information about the
structure of molecules. This method is widely used in organic
chemistry (as well as in medicine, where it is referred to as
MRI). We have a 60 MHz proton FT-NMR, funded in large part by a
grant from the National Science Foundation.
- Infrared Spectroscopy (IR): uses the ability of
chemical bonds to absorb infrared radiation to detect bond types
in molecules. IR spectroscopy is extensively used in organic and
inorganic chemistry. We have a FT-IR, funded by a grant from the
National Science Foundation.
- Atomic Absorption (AA): detects individual chemical
elements by measuring the absorption of light by a
high-temperature vapor. This method is widely used to detect trace
elements such as lead, mercury, cadmium, and others. We have two
AA's, which were donated by industrial labs some years ago.
- Gas Chromatography/Mass Spectrometry (GC/MS): separates
mixtures of volatile compounds, and identifies the individual
compounds based on the masses of the compounds and their
fragments. GC/MS finds wide use in the analysis of mixtures of
organic substances; it can also be used to analyze volatile
inorganic mixtures. We have one GC/MS instrument, funded in large
part by a grant from the National Science Foundation. (We also
have three stand-alone gas chromatographs.)
- High-Pressure Liquid Chromatography (HPLC): separates
mixtures of compounds in solution. This method is widely used in
biology and biotechnology for separating mixtures containing very
small amounts of solutes. We have one HPLC, funded by a grant from
the National Science Foundation.
- High-Pressure Ion Chromatography (HPIC): similar to
HPLC, but separates mixtures of ions in solution. HPIC is used to
analyze mineral and other samples for trace metals and other ions
(nitrates, phosphates, etc.). We have one HPIC, funded by a grant
from the National Institute of Health.
- Capillary Electrophoresis (CE): this is a new
analytical method, similar in its uses to HPLC but more sensitive
and able to deal with smaller amounts of analytes. It is rapidly
becoming the method of choice in the biotechnology industry. We
have one CE, obtained primarily through instructional equipment
funding from the state of California.
In addition, students at City College routinely use a wide range
of more common instruments, including visible and UV-visible
spectrophotometers, pH meters, electronic balances, melting-point
apparatus, voltmeters (multimeters), digital thermometers, and
others. They also have access to two dedicated computer studios which
are equipped with a wide range of educational and analytical
software.
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